MAPKAP Kinase 2 antibody (AA 44-55)

Catalog No. ABIN1590004

This Goat Polyclonal antibody specifically detects MAPKAP Kinase 2 in WB, ELISA and IP. It exhibits reactivity toward Mouse.

Quick Overview for MAPKAP Kinase 2 antibody (AA 44-55) (ABIN1590004)

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MAPKAP Kinase 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-MAPKAP Kinase 2 Antibody

Binding Specificity: AA 44-55

Purpose: MAPKAPK2 (aa44-55)

Sequence: PQFHVKSGLQ IK

Specificity: This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, I

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Alternative Name: MAPKAPK2

Background: MAPKAPK2, mitogen-activated protein kinase-activated protein kinase 2, MAPKAP-K2, MK-2, MK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2

Gene ID: 9261, 17164, 289014

NCBI Accession: NP_004750, NP_116584

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades